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AP Biology Labs
 Molecular Biology & Biotechnology
This lab is a nice quick paper demo on the process of cloning a gene into a plasmid. It reviews the concepts of restriction enzymes, "sticky ends", cloning, and transformation. It is helpful to get a picture in students' heads of the process. Attached to this lab are model DNA sequences representing the puc18 plasmid & the Jellyfish Glo gene sequence. For this exercise, you need to give each student a copy of the puc18 plasmid DNA sequence on strip of white paper & a copy of the Jellyfish Glo gene sequence on a strip of green paper. (V2.0)
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